home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9408a.zip
/
M9480010.TXT
< prev
next >
Wrap
Text File
|
1994-08-09
|
2KB
|
40 lines
Document 0010
DOCN M9480010
TI [Validity of an ELISA test for CD4+ T lymphocyte count and validity of
total lymphocyte count in the assessment of immunodeficiency status in
HIV infection]
DT 9410
AU Loua A; Kestens L; Vanham G; Boel L; Colebunders R; Gigase P;
Departement de Biologie Clinique, CHU Ignace DEEN, Conakry,; Guinee.
SO Ann Soc Belg Med Trop. 1994 Mar;74(1):61-8. Unique Identifier : AIDSLINE
MED/94296163
AB A newly available commercial ELISA (TRAx CD4, T Cell Diagnostics USA)
for enumerating CD4+ T lymphocytes has been evaluated with blood samples
of 105 HIV seropositive and 6 seronegative subjects. Results from the
flow cytometric analysis were used as reference. The sensitivity and
specificity of the ELISA to identify HIV seropositive subjects having
less than 200 CD4+ T lymphocytes/microliters were assessed and studied
using the ROC curve. The reproducibility of the ELISA test was analyzed
on 40 samples. The results of the ELISA correlated well with these of
the flow cytometric analysis (r = 0.79, p < 0.001). However, the ELISA
test tends to overestimate the true CD4 count in HIV seropositives. This
overestimation could not be explained by the aspecific contribution of
monocytic CD4. The threshold for identifying HIV seropositive subjects
with less than 200 CD4+ T lymphocytes with a maximum sensitivity and
specificity was determined with ROC curve and equalled 400 cell
equivalents with the ELISA (sensitivity and specificity were equal to
80%) and 1,450 lymphocytes/microliters with the total absolute
lymphocyte count (sensitivity and specificity were equal to 75%). Using
this curve, a threshold of 300 cell equivalents for the ELISA test and
of 1,100 lymphocytes/microliters for the absolute lymphocyte count was
shown to maximize the specificity (> 95%) without a significant loss of
sensitivity.
DE Comparative Study English Abstract *Enzyme-Linked Immunosorbent Assay
Flow Cytometry Human HIV Infections/*BLOOD *Leukocyte Count
Reproducibility of Results ROC Curve Sensitivity and Specificity *T4
Lymphocytes JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).